Population Genetics

Variation is basis for evolution

            Discontinuous: sexes, blood groups, coloration phases

            Continuous: range of character states: lengths, scales, spines,

            Genotype: genetic content

            Phenotype: expressed genetic traits modified by interaction

            Phenotypic plasticity: expression range of a genotype

            Inducible vs Constitutive genes

            Diploid = 2x copies, locus is gene position of alleles

            homozygous = alleles same; heterozygous, alleles different

Sources of new genetic variations:

            Reassortment / recombination of genes and chromosomes:

                        meiosis, crossing over, transposition

            Mutations: Inheritable change in genetic material

                        Macro mutation: polyploidy, chromosome deletion, large

                                    structural changes that are often lethal

            Micromutation: change in one or few bases within genes:

                        neutral, quasi-neutral mutations

                        radiation/chemical damage

Mutations must become fixed

 

Variation + selective pressures: results in future generations

Passive or directed evolution?

Induced Variation in response to selective pressure

Selective pressures on humans: Eugenetics - Socialism spectrum

Evolution in genetic terms:

            change in the proportion of an expressed gene.

Hardy Weinburg Law

            the gene pool: looking at genes rather than individuals

            can be misleading, since individuals are comprised of many traits

 

For diploid, sexually reproducing organisms, phenotypes genotypes, and genes will approach equilibrium at:

1 = p2 + 2pq + q2

p = frequency of one allele

q = frequency of alternate allele

homozygous = pp or qq

heterozygous = pq

 

Gene frequencies will approach and remain in equilibrium as long as some conditions are met: assumptions of equilibrium

 

            This distribution of alleles gives us a starting point for examining factors that induce changes in gene frequencies.

 

Equilibrium of genes will be maintained if these hold:

1) random mating:

            like to like increases homozygosity: inbreeding depression

            outbreeding increases heterozygosity: Hybrid vigor

2) Mutations do not occur, or the rate and reverse are equal

3) Population is closed:

Immigration and Emigration: selective pressures force particular traits in or out

4) Population size is infinite

5) No Natural Selection:

            directional change of gene frequencies: non-random reproduction

 

Hardy Weinburg allows us to quantify natural selection as a

                        null hyptothesis

Genetic Fitness: a genotype’s contibution to the next generation:

Absolute or Crude Fitness as proportional change F1/P:

B1B1= 0.6; B1B2= 1.0; B2B2 =1.5

Relative genotype fitness: AF/AFmax

B1B1= 0.4; B1B2= .67; B2B2 =1.0

Coefficient of selection s = 1-(AF)

            Can use s in Hardy Weinburg to get change in gene frequency

Dq = change in gene frequency; s= 1/2sq(1-q)

 

Individuals selected against: do not reproduce: Genetic Death

            Proportion of populations carrying negatively selected

                        traits = Genetic Load

 

Nonrandom Reproduction: Evolution

Antibiotics, Insecticides

Pyrethrin Selection: Science 270:1497-1499; 1995

Na channels locus change in tobacco budworm

            Hypothesis: 50 other loci should be homogeneous

                        from gene flow TX to GA

            lower proportion of selective pressure in GA, greater fixation in TX

 

Change in selective pressures

will enhance survival of previously outcompeted or lethal mutations/phenotypes

                        DDT, Antibiotic resistance, etc., sickle cell

Specialized vs simplified; convergence vs divergence,

Chilids and water quality,

 

Genetic flexibility/variation positively associated with fitness,

            vs genetically specialized:  toleration of mutation or extinction

loss of genetic variation a concern: endangered species later

 

Types of Selection

Stabilizing: extremes don’t survive

Disruptive: Ends favored, may result in split

Directional: one end favored

Variability Selection: (Science 273:922; 1996):

            adaptive flexibility correlated with environmental oscillation

            complex specializations as a result of temporal disparities in fitness

Constant vs Periodic selection: punctuated equilibrium theory

            Meteor/asteroid impact theories: mass extinction events

            Guppies, Bacteria

 

Group and Kin Selection (unit of selection)

Altruism

Raising fitness of another a cost of the individual’s fitness

Such genes should be selected against, yet can be found:

            Warning calls, warning coloration

True Altruism may be rare.

Wynne-Edwards: reproductive restraint: staying below K

   Subpopulations that exceed K will starve,

can be replaced by those who have group traits keeping population in check

How could an altruistic trait survive? Fig. 19.11

 

Apparent altruism

Kin Selection: helping those with related genetic makeup

            Table 5-9: shared genes: three brothers or nine cousins

Cooperative breeding behavior

Helping: non-breeding individuals in a group:

            Eusocial species: reproductive altruism

            Tropical wrens, Florida Scrub Jays, Red Cockaded Woodpeckers:

                        Jackals Fig 19.14

 

Inbreeding

Increase in Homozygosity with inbreeding Fig. 19.16

            Frequency of Alleles remains the same

                        Frequency of homozygosity increases

                        Genetic variation within individuals becomes

variation between individuals

Inbreeding Depression/Hybrid Vigor

            Increased expression of recessive deleterious mutations

                        May purge deleterious traits or cause extinction.

            Outbreeding depression: disruption of local population adaptations

 

 

Genetic Drift

Loss of alleles by random sampling in gametes

Larger the population,

more likely the F1 generation will have all the same alleles

large populations as metapopulations: genetically small popuations

Fluctuations in allele compostion of subpopulations is Genetic Drift

Like Inbreeding (non-random mating)

Genetic Drift (loss of alleles from random mating) increases homozygosity

            Both may be acting in subpopulations

 

Effective Population Size

Members of a population contributing to gametes to successful reproduction

 Skewed Sex ratios in polygamous species decreases EP

 

Bottlenecks and Founder Effects

 

Minimum Viable Population MVP